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Literature summary extracted from

  • Liu, J.; Zhou, G.Q.; Strabym K.B.
    Caenorhabditis elegans ZC376.5 encodes a tRNA (m2/2G(26))dimethyltransferance in which (246)arginine is important for the enzyme activity (1999), Gene, 226, 73-81.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.216 expression in Escherichia coli Caenorhabditis elegans

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.216 K290A mutant enzyme loses 56% of its methylation activity Saccharomyces cerevisiae
2.1.1.216 R246A more than 97% loss of activity Caenorhabditis elegans
2.1.1.216 R246G mutation eliminates the methylation activity Caenorhabditis elegans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.216 58400
-
 calculated from sequence Caenorhabditis elegans
2.1.1.216 59000
-
 SDS-PAGE Caenorhabditis elegans

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.216 Caenorhabditis elegans Q23270
-
-
2.1.1.216 Saccharomyces cerevisiae P15565
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.216
-
 Caenorhabditis elegans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Saccharomyces cerevisiae 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNA wild-type enzyme produces 88% N2-dimethylguanine26 tRNA and 12% N2-methylguanine26 in tRNA. The Caenorhabditis elegans tRNA(m22G26)dimethyltransferase efficiently recognized the Escherichia coli tRNA as a substrate in vivo, and that the enzyme is capable of both mono- and dimethylating G26 in this tRNA Caenorhabditis elegans 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Caenorhabditis elegans S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
-
 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
-
 Caenorhabditis elegans S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.216 Trm1p
-
 Saccharomyces cerevisiae
2.1.1.216 tRNA(m22G26)dimethyltransferase
-
 Saccharomyces cerevisiae
2.1.1.216 tRNA(m22G26)dimethyltransferase
-
 Caenorhabditis elegans
2.1.1.216 ZC376.5
-
 Caenorhabditis elegans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.216 Caenorhabditis elegans calculated from sequence
-
 8.7